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Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells
Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenche...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757292/ https://ncbi.nlm.nih.gov/pubmed/33377051 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100157 |
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