Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells

Aquaporins (AQPs) are membrane channel proteins that facilitate the movement of water down osmotic gradients across biological membranes. This protocol allows measurements of AQP-mediated water transport across the plasma membrane of live mammalian cells. Calcein is a fluorescent dye that is quenche...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:STAR Protoc
Autors principals: Kitchen, Philip, Salman, Mootaz M., Abir-Awan, Mohammed, Al-Jubair, Tamim, Törnroth- Horsefield, Susanna, Conner, Alex C., Bill, Roslyn M.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2020
Matèries:
Protocol
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757292/
https://ncbi.nlm.nih.gov/pubmed/33377051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100157
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars