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Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells
Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative...
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| Published in: | STAR Protoc |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Elsevier
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757111/ https://ncbi.nlm.nih.gov/pubmed/33377022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100128 |
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