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Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells
Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative...
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| Опубликовано в: : | STAR Protoc |
|---|---|
| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Elsevier
2020
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757111/ https://ncbi.nlm.nih.gov/pubmed/33377022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100128 |
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