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Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells

Quiescent muscle stem cells, also called satellite cells (SCs), are essential for muscle regeneration. However, quiescent SCs are quickly activated during fluorescence-activated cell sorting (FACS) isolation. Here, we present an optimized protocol to isolate quiescent muscle stem cells from fixative...

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Bibliografische gegevens
Gepubliceerd in:STAR Protoc
Hoofdauteurs: Yue, Lu, Cheung, Tom H.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757111/
https://ncbi.nlm.nih.gov/pubmed/33377022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100128
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