Making Droplet-Embedded Vesicles to Model Cellular Lipid Droplets

We present a reproducible protocol to prepare droplet-embedded vesicles (DEVs) consisting of an oil droplet embedded within a phospholipid bilayer. This model system mimics a cellular lipid droplet (LD) in physical contact with the endoplasmic reticulum (ER) bilayer. It has the advantage that the li...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Chorlay, Aymeric, Santinho, Alexandre, Thiam, Abdou Rachid
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757013/
https://ncbi.nlm.nih.gov/pubmed/33377012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100116
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