Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes

Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Commonly ap...

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Podrobná bibliografie
Vydáno v:STAR Protoc
Hlavní autoři: Oeckl, Josef, Bast-Habersbrunner, Andrea, Fromme, Tobias, Klingenspor, Martin, Li, Yongguo
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757011/
https://ncbi.nlm.nih.gov/pubmed/33377014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100118
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