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Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
BACKGROUND: Thrombin activates fibrinogen and binds the fibrin E-domain (K(d) ~ 2.8 μM) and the splice variant γ’-domain (K(d) ~ 0.1 μM). We investigated if the loading of D-Phe-Pro-Arg-chloromethylketone inhibited thrombin (PPACK-thrombin) onto fibrin could enhance fibrin stability. METHODS: A 384-...
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| Izdano u: | Biochim Biophys Acta Gen Subj |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2020
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7752828/ https://ncbi.nlm.nih.gov/pubmed/33276061 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2020.129805 |
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