Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

We describe the optimisation of a simplified sample preparation method which permits rapid and direct detection of SARS-CoV-2 RNA within saliva, using reverse-transcription loop-mediated isothermal amplification (RT-LAMP). Treatment of saliva samples prior to RT-LAMP by dilution 1:1 in Mucolyse™, fo...

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Bibliografske podrobnosti
izdano v:J Virol Methods
Main Authors: Howson, Emma L.A., Kidd, Stephen P., Armson, Bryony, Goring, Alice, Sawyer, Jason, Cassar, Claire, Cross, David, Lewis, Tom, Hockey, Jess, Rivers, Samantha, Cawthraw, Saira, Banyard, Ashley, Anderson, Paul, Rahou, Sabah, Andreou, Michael, Morant, Nick, Clark, Duncan, Walsh, Charlotte, Laxman, Shailen, Houghton, Rebecca, Slater-Jefferies, Joanne, Costello, Paula, Brown, Ian, Cortes, Nicholas, Godfrey, Keith M., Fowler, Veronica L.
Format: Artigo
Jezik:Inglês
Izdano: Elsevier B.V. 2021
Teme:
Short Communication
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7750029/
https://ncbi.nlm.nih.gov/pubmed/33358911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2020.114048
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