Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

We describe the optimisation of a simplified sample preparation method which permits rapid and direct detection of SARS-CoV-2 RNA within saliva, using reverse-transcription loop-mediated isothermal amplification (RT-LAMP). Treatment of saliva samples prior to RT-LAMP by dilution 1:1 in Mucolyse™, fo...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Howson, Emma L.A., Kidd, Stephen P., Armson, Bryony, Goring, Alice, Sawyer, Jason, Cassar, Claire, Cross, David, Lewis, Tom, Hockey, Jess, Rivers, Samantha, Cawthraw, Saira, Banyard, Ashley, Anderson, Paul, Rahou, Sabah, Andreou, Michael, Morant, Nick, Clark, Duncan, Walsh, Charlotte, Laxman, Shailen, Houghton, Rebecca, Slater-Jefferies, Joanne, Costello, Paula, Brown, Ian, Cortes, Nicholas, Godfrey, Keith M., Fowler, Veronica L.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier B.V. 2021
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7750029/
https://ncbi.nlm.nih.gov/pubmed/33358911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2020.114048
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