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High-throughput quantification of ochronotic pigment formation in Escherichia coli to evaluate the potency of human 4-hydroxyphenylpyruvate dioxygenase inhibitors in multi-well format

4-hydroxyphenylpyruvate dioxygenase (HPD) is a key enzyme in the catabolism of tyrosine and therefore of great importance as a drug target to treat tyrosine-related inherited metabolic disorders (TIMD). Inhibition of this enzyme is therapeutically applied to prevent accumulation of toxic metabolites...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Neuckermans, Jessie, Lequeue, Sien, Mertens, Alan, Branson, Steven, Schwaneberg, Ulrich, De Kock, Joery
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7749435/
https://ncbi.nlm.nih.gov/pubmed/33365261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.101181
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