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Simple in vitro assay of Arf GAPs and preparation of Arf proteins as substrates

Defining the interaction of Arf GAPs with specific Arfs is important for understanding their functions in the endocytic system. Cell-based approaches have been valuable for identifying Arfs and Arf GAPs active in the endocytic compartment; however, the cell-based assays have some limitations in esta...

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Bibliografske podrobnosti
izdano v:Methods Cell Biol
Main Authors: Chen, Pei-Wen, Jian, Xiaoying, Luo, Ruibai, Randazzo, Paul A.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7743909/
https://ncbi.nlm.nih.gov/pubmed/26360029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2015.03.021
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