Evaluation of methods for the reduction of contaminating host reads when performing shotgun metagenomic sequencing of the milk microbiome

Shotgun metagenomic sequencing is a valuable tool for the taxonomic and functional profiling of microbial communities. However, this approach is challenging in samples, such as milk, where a low microbial abundance, combined with high levels of host DNA, result in inefficient and uneconomical sequen...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Yap, Min, Feehily, Conor, Walsh, Calum J., Fenelon, Mark, Murphy, Eileen F., McAuliffe, Fionnuala M., van Sinderen, Douwe, O’Toole, Paul W., O’Sullivan, Orla, Cotter, Paul D.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7728742/
https://ncbi.nlm.nih.gov/pubmed/33303873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-78773-6
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