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Assessment of Cellular Oxidation Using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Measuring the intracellular oxidation/reduction balance provides an overview of the physiological and/or pathophysiological redox status of an organism. Thiols are especially important for illuminating the redox status of cells via their reduced dithiol and oxidized disulfide ratios. Engineered cyst...

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Publicado en:J Vis Exp
Main Authors: Aliyev, Alev Tascioglu, LoBianco, Francesca, Krager, Kimberly J., Aykin-Burns, Nukhet
Formato: Artigo
Idioma:Inglês
Publicado: 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7725482/
https://ncbi.nlm.nih.gov/pubmed/32628158
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61229
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