Production of isoform-specific knockdown/knockout Madin–Darby canine kidney epithelial cells using CRISPR/Cas9

CRISPR-Cas9 gene editing has made it possible to specifically edit genes in a myriad of target cells. Here, a method for isoform-specific editing and clonal selection in Madin-Darby canine kidney (MDCK) epithelial cells is described in detail. This approach was used to address a long-standing questi...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Readler, James M., AlKahlout, Amal S., Kolawole, Abimbola O., Excoffon, Katherine J.D.A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Method Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7718483/
https://ncbi.nlm.nih.gov/pubmed/33304833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.101149
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