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3D projection electrophoresis for single-cell immunoblotting

Immunoassays and mass spectrometry are powerful single-cell protein analysis tools; however, interfacing and throughput bottlenecks remain. Here, we introduce three-dimensional single-cell immunoblots to detect both cytosolic and nuclear proteins. The 3D microfluidic device is a photoactive polyacry...

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Détails bibliographiques
Publié dans:Nat Commun
Auteurs principaux: Grist, Samantha M., Mourdoukoutas, Andoni P., Herr, Amy E.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7718224/
https://ncbi.nlm.nih.gov/pubmed/33277486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-19738-1
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