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An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures
In this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. Th...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | Methods Protoc |
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| Κύριοι συγγραφείς: | , , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
MDPI
2020
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7712542/ https://ncbi.nlm.nih.gov/pubmed/33066602 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3040069 |
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