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An Efficient Method for Isolation of Plasmid DNA for Transfection of Mammalian Cell Cultures

In this article, we present several protocols that describe the steps from cloning and obtaining a large amount of pure plasmid DNA to generation of lentiviruses based on these constructs. The protocols have been worked out on human cell culture HEK293T but can be adapted for other cell cultures. Th...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Methods Protoc
Prif Awduron: Kachkin, Daniel V., Khorolskaya, Julia I., Ivanova, Julia S., Rubel, Aleksandr A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: MDPI 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7712542/
https://ncbi.nlm.nih.gov/pubmed/33066602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3040069
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