ProAlanase is an Effective Alternative to Trypsin for Proteomics Applications and Disulfide Bond Mapping

Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on t...

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Veröffentlicht in:Mol Cell Proteomics
Hauptverfasser: Samodova, Diana, Hosfield, Christopher M., Cramer, Christian N., Giuli, Maria V., Cappellini, Enrico, Franciosa, Giulia, Rosenblatt, Michael M., Kelstrup, Christian D., Olsen, Jesper V.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2020
Schlagworte:
Technological Innovation and Resources
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7710147/
https://ncbi.nlm.nih.gov/pubmed/33020190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.TIR120.002129
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