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Quantitative PCR assays to detect whales, rockfish, and common murre environmental DNA in marine water samples of the Northeastern Pacific

Monitoring aquatic species by identification of environmental DNA (eDNA) is becoming more common. To obtain quantitative eDNA datasets for individual species, organism-specific quantitative PCR (qPCR) assays are required. Here, we present detailed methodology of qPCR assay design and testing, includ...

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Publicat a:PLoS One
Autors principals: Andruszkiewicz, Elizabeth A., Yamahara, Kevan M., Closek, Collin J., Boehm, Alexandria B.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7710076/
https://ncbi.nlm.nih.gov/pubmed/33264323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0242689
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