NMR Spectroscopic Characterization of the C‐Mannose Conformation in a Thrombospondin Repeat Using a Selective Labeling Approach

Despite the great interest in glycoproteins, structural information reporting on conformation and dynamics of the sugar moieties are limited. We present a new biochemical method to express proteins with glycans that are selectively labeled with NMR‐active nuclei. We report on the incorporation of (1...

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Publicat a:Angew Chem Int Ed Engl
Autors principals: Jonker, Hendrik R. A., Saxena, Krishna, Shcherbakova, Aleksandra, Tiemann, Birgit, Bakker, Hans, Schwalbe, Harald
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2020
Matèries:
Research Articles
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7692951/
https://ncbi.nlm.nih.gov/pubmed/32745319
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.202009489
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