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In-gel fluorescence detection by DNA polymerase elongation

Fluorescence-based DNA readouts are increasingly important in biological research, owing to enhanced analytical sensitivity and multiplexing capability. In this study, we characterize an in-gel polymerase elongation process to understand the reaction kinetics and transport limitations, and we evalua...

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Detalhes bibliográficos
Publicado no:APL Bioeng
Main Authors: Moss, Alden C., Herr, Amy E.
Formato: Artigo
Idioma:Inglês
Publicado em: AIP Publishing LLC 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7680656/
https://ncbi.nlm.nih.gov/pubmed/33263097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/5.0021149
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