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Using RNA Sequencing and Spike-in RNAs to Measure Intracellular Abundance of lncRNAs and mRNAs

Long noncoding RNAs (lncRNAs) play essential roles in normal physiology and in disease but their mechanisms of action can be challenging to identify. For mechanistic studies, it is often useful to know a lncRNA’s intracellular abundance, i.e., approximately how many molecules of the lncRNA are prese...

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Publicat a:Bio Protoc
Autors principals: Schertzer, Megan D., Murvin, McKenzie M., Calabrese, J. Mauro
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7668557/
https://ncbi.nlm.nih.gov/pubmed/33204768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3772
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