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A rapid RNA extraction method from oil palm tissues suitable for reverse transcription quantitative real-time PCR (RT-qPCR)

Cetyltrimethylammonium bromide (CTAB) is the preferred detergent in RNA extraction of oil palm tissues. However, the CTAB-based protocol is time-consuming. In this study, a combination of the CTAB-based method and silica-based purification reduced the extraction time from two days to five hours. Qua...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:3 Biotech
Hauptverfasser: Badai, Siti Suriawati, Rasid, Omar Abd, Parveez, Ghulam Kadir Ahmad, Masani, Mat Yunus Abdul
Format: Artigo
Sprache:Inglês
Veröffentlicht: Springer International Publishing 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7661601/
https://ncbi.nlm.nih.gov/pubmed/33214977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02514-9
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