An automated workflow to screen alkene reductases using high-throughput thin layer chromatography

BACKGROUND: Synthetic biology efforts often require high-throughput screening tools for enzyme engineering campaigns. While innovations in chromatographic and mass spectrometry-based techniques provide relevant structural information associated with enzyme activity, these approaches can require cost...

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Veröffentlicht in:Biotechnol Biofuels
Hauptverfasser: Garabedian, Brett M., Meadows, Corey W., Mingardon, Florence, Guenther, Joel M., de Rond, Tristan, Abourjeily, Raya, Lee, Taek Soon
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2020
Schlagworte:
Methodology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7653764/
https://ncbi.nlm.nih.gov/pubmed/33292503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-020-01821-w
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