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Optimising total RNA quality and quantity by phenol-chloroform extraction method from human visceral adipose tissue: A standardisation study

A multitude of challenges is faced during RNA extraction from human visceral adipose tissue (VAT) due to its atypical nature and a dearth of existing literature. Our study provides a convenient and inexpensive manual method using TRIzol reagent for the reproducible recovery of intact RNA from sparse...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Roy, Dipayan, Tomo, Sojit, Modi, Anupama, Purohit, Purvi, Sharma, Praveen
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7653057/
https://ncbi.nlm.nih.gov/pubmed/33204654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.101113
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