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Polymerization of Bacillus subtilis MreB on a lipid membrane reveals lateral co-polymerization of MreB paralogs and strong effects of cations on filament formation

BACKGROUND: MreB is a bacterial ortholog of actin and forms mobile filaments underneath the cell membrane, perpendicular to the long axis of the cell, which play a crucial role for cell shape maintenance. We wished to visualize Bacillus subtilis MreB in vitro and therefore established a protocol to...

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Publicado en:BMC Mol Cell Biol
Autores principales: Dersch, Simon, Reimold, Christian, Stoll, Joshua, Breddermann, Hannes, Heimerl, Thomas, Defeu Soufo, Hervé Joel, Graumann, Peter L.
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7641798/
https://ncbi.nlm.nih.gov/pubmed/33148162
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12860-020-00319-5
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