Bacterial genome editing by coupling Cre-lox and CRISPR-Cas9 systems

The past decade has been a golden age for microbiology, marked by the discovery of an unprecedented increase in the number of novel bacterial species. Yet gaining biological knowledge of those organisms has not kept pace with sequencing efforts. To unlock this genetic potential there is an urgent ne...

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Bibliographic Details
Published in:PLoS One
Main Authors: Liu, Hualan, Robinson, David S., Wu, Zong-Yen, Kuo, Rita, Yoshikuni, Yasuo, Blaby, Ian K., Cheng, Jan-Fang
Format: Artigo
Language:Inglês
Published: Public Library of Science 2020
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7641437/
https://ncbi.nlm.nih.gov/pubmed/33147260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0241867
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