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Rapid poxvirus engineering using CRISPR/Cas9 as a selection tool
In standard uses of CRISPR/Cas9 technology, the cutting of genomes and their efficient repair are considered to go hand-in-hand to achieve desired genetic changes. This includes the current approach for engineering genomes of large dsDNA viruses. However, for poxviruses we show that Cas9-guide RNA c...
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| 發表在: | Commun Biol |
|---|---|
| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Nature Publishing Group UK
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7641209/ https://ncbi.nlm.nih.gov/pubmed/33144673 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-020-01374-6 |
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