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Very Fast CRISPR on Demand
CRISPR-Cas systems provide versatile tools for programmable genome editing. Here, we developed a caged RNA strategy that allows Cas9 to bind DNA but not cleave until light-induced activation. This approach, referred to as very fast CRISPR, creates doublestrand-breaks (DSBs) at submicron and seconds...
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| Publicado no: | Science |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7608738/ https://ncbi.nlm.nih.gov/pubmed/32527834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aay8204 |
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