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Purification, characterization and anticancer evaluation of l-methioninase from Trichoderma harzianum

The Trichoderma harzianum l-methioninase was purified 7.15-fold with a recovery of 47.9% and the specific activity of 74.4 U/mg of protein. The purified enzyme has an apparent molecular mass of 48 kDa on SDS-PAGE and exhibited maximum activity at pH 8 and 35 °C. The enzyme was catalytically stable b...

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Vydáno v:3 Biotech
Hlavní autoři: Salim, Nisha, Santhiagu, A., Joji, K.
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer International Publishing 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7606426/
https://ncbi.nlm.nih.gov/pubmed/33163320
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02494-w
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