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Cloning short DNA into plasmids by one‐step PCR
BACKGROUND: Plasmid construction of small fragments of interest (such as insertion of small fragment marker genes, expression of shRNA, siRNA, etc) is the basis of many biomolecular experiments. Here, we describe a method to clone short DNA into vectors by polymerase chain reaction (PCR), named one‐...
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| Publicado en: | Thorac Cancer |
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| Autores principales: | , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
John Wiley & Sons Australia, Ltd
2020
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7605993/ https://ncbi.nlm.nih.gov/pubmed/33015950 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1759-7714.13660 |
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