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Heat-Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections

The development of heat-induced antigen (epitope) retrieval (HIER) technologies has led to dramatic improvements in our ability to detect antigens in formalin fixed, archival tissues. Paradoxically, wet heat treatment at temperatures greater than 95°C in appropriate buffer solutions can reconstitute...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Krenacs, Laszlo, Krenacs, Tibor, Stelkovics, Eva, Raffeld, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7604828/
https://ncbi.nlm.nih.gov/pubmed/20012825
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-324-0_14
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