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Isobaric Matching between Runs and Novel PSM-Level Normalization in MaxQuant Strongly Improve Reporter Ion-Based Quantification

[Image: see text] Isobaric labeling has the promise of combining high sample multiplexing with precise quantification. However, normalization issues and the missing value problem of complete n-plexes hamper quantification across more than one n-plex. Here, we introduce two novel algorithms implement...

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Detalhes bibliográficos
Publicado no:J Proteome Res
Main Authors: Yu, Sung-Huan, Kyriakidou, Pelagia, Cox, Jürgen
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2020
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7586393/
https://ncbi.nlm.nih.gov/pubmed/32892627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.0c00209
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