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Fluorescence lifetime imaging reveals heterogeneous functional distribution of eGFP expressed in Xenopus oocytes

The oocytes from Xenopus laevis are well known for their polarity, presenting a distinct animal and vegetal pole. Other heterogeneities are less known. To study the heterogeneity of the Xenopus oocyte, we expressed eGFP and analyzed the protein distribution with fluorescence lifetime microscopy. The...

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Detalhes bibliográficos
Publicado no:Methods Appl Fluoresc
Main Authors: Zeng, Steven L, Grabowska, Dorota, Shahverdi, Kiana, Sudlow, Leland C, Achilefu, Samuel, Berezin, Mikhail Y
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7584348/
https://ncbi.nlm.nih.gov/pubmed/31658452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/2050-6120/ab51f8
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