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Methodology for Whole Mount and Fluorescent RNA in situ Hybridization in Echinoderms: Single, Double, and Beyond.

Identifying the location of a specific RNA in a cell, tissue, or embryo is essential to understand its function. Here we use echinoderm embryos to demonstrate the power of fluorescence in situ RNA hybridizations to localize sites of specific RNA accumulation in whole mount embryo applications. We ad...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Perillo, Margherita, Paganos, Periklis, Spurrell, Maxwell, Arnone, Maria I., Wessel, Gary M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7583651/
https://ncbi.nlm.nih.gov/pubmed/33074542
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0974-3_12
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