High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing

Implementation of gene editing technologies such as CRISPR/Cas9 in the manufacture of novel cell-based therapeutics has the potential to enable highly-targeted, stable, and persistent genome modifications without the use of viral vectors. Electroporation has emerged as a preferred method for deliver...

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Publicado no:Sci Rep
Main Authors: Lissandrello, Charles A., Santos, Jose A., Hsi, Peter, Welch, Michaela, Mott, Vienna L., Kim, Ernest S., Chesin, Jordan, Haroutunian, Nerses J., Stoddard, Aaron G., Czarnecki, Andrew, Coppeta, Jonathan R., Freeman, Daniel K., Flusberg, Deborah A., Balestrini, Jenna L., Tandon, Vishal
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7582186/
https://ncbi.nlm.nih.gov/pubmed/33093518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73755-0
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