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Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons

This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. Th...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Peitz, Michael, Krutenko, Tamara, Brüstle, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580116/
https://ncbi.nlm.nih.gov/pubmed/33111086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100038
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