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Protocol for the Standardized Generation of Forward Programmed Cryopreservable Excitatory and Inhibitory Forebrain Neurons
This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. Th...
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Izdano u: | STAR Protoc |
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Glavni autori: | , , |
Format: | Artigo |
Jezik: | Inglês |
Izdano: |
Elsevier
2020
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Teme: | |
Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580116/ https://ncbi.nlm.nih.gov/pubmed/33111086 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100038 |
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