Protocol for Imaging of Mitoflashes in Live Cardiomyocytes

We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration fu...

Full description

Saved in:
Bibliographic Details
Published in:STAR Protoc
Main Authors: Li, Anqi, Qin, Yuan, Gao, Meng, Jiang, Wenting, Liu, Bilin, Tian, Xiangang, Gong, Guohua
Format: Artigo
Language:Inglês
Published: Elsevier 2020
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580095/
https://ncbi.nlm.nih.gov/pubmed/33111128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100101
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items