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Protocol for Imaging of Mitoflashes in Live Cardiomyocytes
We describe a protocol for imaging a mitochondrial fluorescence transient increase event (Mitoflash) in live cardiomyocytes using a confocal microscope. Mitoflash, detected by mitochondria-targeted circularly permuted fluorescent protein (mt-cpYFP), can be used to assess mitochondrial respiration fu...
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| Gepubliceerd in: | STAR Protoc |
|---|---|
| Hoofdauteurs: | , , , , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Elsevier
2020
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580095/ https://ncbi.nlm.nih.gov/pubmed/33111128 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100101 |
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