Protocol for Quantitative Analysis of Synaptic Vesicle Clustering in Axons of Cultured Neurons

Clustering of synaptic vesicles along the neuronal axons is a critical mechanism underpinning proper synaptic transmission. Here, we provide a detailed protocol for analyzing the distribution of synaptic vesicles in presynaptic boutons of cultured neurons. The protocol covers preparation of cultured...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Um, Ji Won, Han, Kyung Ah, Choi, Se-Young, Ko, Jaewon
Format: Artigo
Language:Inglês
Published: Elsevier 2020
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580093/
https://ncbi.nlm.nih.gov/pubmed/33111124
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100095
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