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Single-Cell Isolation from Regenerating Murine Muscles for RNA-Sequencing Analysis

Single-cell RNA sequencing (scRNA-seq) is a powerful technique for deconvoluting and clustering thousands of otherwise intermingled cells based on their gene expression. Here, we present a complete protocol for the unbiased evaluation of regenerating murine skeletal muscle using scRNA-seq. The skele...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:STAR Protoc
Hauptverfasser: Oprescu, Stephanie N., Yue, Feng, Kuang, Shihuan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580090/
https://ncbi.nlm.nih.gov/pubmed/33111097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100051
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