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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Primary myoblasts are undifferentiated proliferating precursors of skeletal muscle. They can be cultured and studied as muscle precursors or induced to differentiate into later stages of muscle development. The protocol provided here describes a robust method for the isolation and culture of a highl...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Vaughan, Megan, Lamia, Katja A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7577426/
https://ncbi.nlm.nih.gov/pubmed/31680669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60310
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