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Semi-Continuous Flow Biocatalysis with Affinity Co-Immobilized Ketoreductase and Glucose Dehydrogenase

The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Immobilization of these two enzymes was performed via affinity interaction between His-tagged enzymes (six histidine residues on the N-terminus of the protein) and ag...

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Détails bibliographiques
Publié dans:Molecules
Auteurs principaux: Plž, Michal, Petrovičová, Tatiana, Rebroš, Martin
Format: Artigo
Langue:Inglês
Publié: MDPI 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7570937/
https://ncbi.nlm.nih.gov/pubmed/32961948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules25184278
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