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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction

Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol(®) reagent produces a protein pellet that is extremely difficult to solubilize. In res...

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Detalhes bibliográficos
Publicado no:Biotechniques
Main Authors: Wen, Yuan, Vechetti, Ivan J, Valentino, Taylor R, McCarthy, John J
Formato: Artigo
Idioma:Inglês
Publicado em: Future Science Ltd 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7566772/
https://ncbi.nlm.nih.gov/pubmed/32777951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/btn-2020-0083
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