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Optimized DNA extraction and purification method for characterization of bacterial and fungal communities in lung tissue samples
Human lungs harbor a scarce microbial community, requiring to develop methods to enhance the recovery of nucleic acids from bacteria and fungi, leading to a more efficient analysis of the lung tissue microbiota. Here we describe five extraction protocols including pre-treatment, bead-beating and/or...
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| Publicat a: | Sci Rep |
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| Autors principals: | , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group UK
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7562954/ https://ncbi.nlm.nih.gov/pubmed/33060634 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-74137-2 |
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