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Selection of endogenous control genes for normalising gene expression data derived from formalin-fixed paraffin-embedded tumour tissue

Quantitative real time polymerase chain reaction (qPCR) data are normalised using endogenous control genes. We aimed to: (1) demonstrate a pathway to identify endogenous control genes for qPCR analysis of formalin-fixed paraffin-embedded (FFPE) tissue using bladder cancer as an exemplar; and (2) exa...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Smith, Tim A. D., AbdelKarem, Omneya A., Irlam-Jones, Joely J., Lane, Brian, Valentine, Helen, Bibby, Becky A. S., Denley, Helen, Choudhury, Ananya, West, Catharine M. L.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7560892/
https://ncbi.nlm.nih.gov/pubmed/33057113
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-74380-7
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