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Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes

Indirect co-culture models with osteoclasts including oral cell lines may be influenced by M-CSF and RANKL in the common cell medium. Therefore, we investigated the viability and proliferation of osteoblasts (OB), fibroblasts (FB) and oral keratinocytes (OK) under stratified medium modification and...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Steller, Daniel, Scheibert, Alexandra, Sturmheit, Tabea, Hakim, Samer G.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7544897/
https://ncbi.nlm.nih.gov/pubmed/33033302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73941-0
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