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Development of an Online 2D Ultrahigh-Pressure Nano-LC System for High-pH and Low-pH Reversed Phase Separation in Top-Down Proteomics

The development of novel high-resolution separation techniques is crucial for advancing the complex sample analysis necessary for high-throughput top-down proteomics. Recently, our group developed an offline 2D high-pH RPLC/low-pH RPLC separation method and demonstrated good orthogonality between th...

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Publicado en:Anal Chem
Autores principales: Wang, Zhe, Yu, Dahang, Cupp-Sutton, Kellye A., Liu, Xiaowen, Smith, Kenneth, Wu, Si
Formato: Artigo
Lenguaje:Inglês
Publicado: 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7544661/
https://ncbi.nlm.nih.gov/pubmed/32857493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.0c03395
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