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Creation of Golden Gate constructs for gene doctoring
BACKGROUND: Gene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is cleaved in vivo to generate linear DNA fragments suitable for recombination. The us...
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| Published in: | BMC Biotechnol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
BioMed Central
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7542709/ https://ncbi.nlm.nih.gov/pubmed/33028286 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-020-00648-5 |
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