Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish

To determine malachite green (MG) and its major metabolite, leucomalachite green (LMG) residual levels in tilapia fish, chemiluminescent enzyme immunoassay (CLEIA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. At first, V(H) and V(L) gene se...

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Bibliografiske detaljer
Udgivet i:	Food Chem
Main Authors:	Dong, Jiexian, Li, Zhenfeng, Wang, Yu, Jin, Maojun, Shen, Yudong, Xu, Zhenlin, El-Aty, A. M. Abd, Gee, Shirley J., Hammock, Bruce D., Sun, Yuanming, Wang, Hong
Format:	Artigo
Sprog:	Inglês
Udgivet:	2020
Fag:	Article
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC7541715/ https://ncbi.nlm.nih.gov/pubmed/32799164 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.foodchem.2020.127780
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Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish

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