Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish

To determine malachite green (MG) and its major metabolite, leucomalachite green (LMG) residual levels in tilapia fish, chemiluminescent enzyme immunoassay (CLEIA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. At first, V(H) and V(L) gene se...

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Xehetasun bibliografikoak
Argitaratua izan da:Food Chem
Egile Nagusiak: Dong, Jiexian, Li, Zhenfeng, Wang, Yu, Jin, Maojun, Shen, Yudong, Xu, Zhenlin, El-Aty, A. M. Abd, Gee, Shirley J., Hammock, Bruce D., Sun, Yuanming, Wang, Hong
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2020
Gaiak:
Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC7541715/
https://ncbi.nlm.nih.gov/pubmed/32799164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.foodchem.2020.127780
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