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Reprogramming Urine‐Derived Cells using Commercially Available Self‐Replicative RNA and a Single Electroporation
We describe a protocol for efficient generation of human‐induced pluripotent stem cells (hiPSCs) from urine‐derived cells (UDCs) obtained from adult donors using self‐replicative RNA containing the reprogramming factors OCT3/4, SOX2, KLF4, GLIS1, and c‐MYC (ReproRNA‐OKSGM). After electroporation, tr...
Gorde:
| Argitaratua izan da: | Curr Protoc Stem Cell Biol |
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| Egile Nagusiak: | , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
John Wiley and Sons Inc.
2020
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7540473/ https://ncbi.nlm.nih.gov/pubmed/32956580 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.124 |
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