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Improving the Expression of Human Granulocyte Colony Stimulating Factor in Escherichia coli by Reducing the GC-content and Increasing mRNA Folding Free Energy at 5’-Terminal End

Purpose: Strategy for improving the production of biopharmaceutical protein continues to develop due to increasing market demand. Human granulocyte colony stimulating factor (hG-CSF) is one of biopharmaceutical proteins that has many applications, and easily produced in Escherichia coli expression s...

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Bibliografske podrobnosti
izdano v:Adv Pharm Bull
Main Authors: Dewi, Kartika Sari, Fuad, Asrul Muhamad
Format: Artigo
Jezik:Inglês
Izdano: Tabriz University of Medical Sciences 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7539321/
https://ncbi.nlm.nih.gov/pubmed/33072539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.34172/apb.2020.073
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