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Lost in translation: the quest for Nitrosomonas cluster 7‐specific amoA primers and TaqMan probes

The choice of primer and TaqMan probes to quantify ammonia‐oxidizing bacteria (AOB) in environmental samples is of crucial importance. The re‐evaluation of primer pairs based on current genomic sequences used for quantification of the amoA gene revealed (1) significant misrepresentations of the AOB...

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Bibliografiske detaljer
Udgivet i:Microb Biotechnol
Main Authors: Orschler, Laura, Agrawal, Shelesh, Lackner, Susanne
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7533338/
https://ncbi.nlm.nih.gov/pubmed/32686322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13627
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