Characterization and mitigation of fragmentation enzyme-induced dual stranded artifacts

High-throughput short-read sequencing relies on fragmented DNA for optimal sampling of input nucleic acid. Several vendors now offer proprietary enzyme cocktails as a cheaper and more streamlined method of fragmentation when compared to acoustic shearing. We have discovered that these enzymes induce...

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Publicado en:NAR Genom Bioinform
Autores principales: Gregory, Thomas, Ngankeu, Apollinaire, Orwick, Shelley, Kautto, Esko A, Woyach, Jennifer A, Byrd, John C, Blachly, James S
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7531576/
https://ncbi.nlm.nih.gov/pubmed/33043294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nargab/lqaa070
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