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Quantitative method for estimating stain density in electron microscopy of conventionally prepared biological specimens

Stain density is an important parameter for optimizing the quality of ultrastructural data obtained from several types of 3D electron microscopy techniques, including serial block-face electron microscopy (SBEM), and focused ion beam scanning electron microscopy (FIB-SEM). Here, we show how some str...

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Detalhes bibliográficos
Publicado no:J Microsc
Main Authors: Fera, Andrea, He, Qianping, Zhang, Guofeng, Leapman, Richard D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7530943/
https://ncbi.nlm.nih.gov/pubmed/31994199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jmi.12865
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